Culturefuge
Alfa Laval Culturefuge es una gama de centrífugas de discos y separadores biofarmacéuticos (o sistemas de separación completos) únicos, desarrollados especialmente para la recolección cuidadosa de cultivos de células de mamíferos, células microbianas y proteínas precipitadas. La aceleración de alimentación suave y eficiente resultante evita la destrucción de las membranas celulares sensibles al cizallamiento, lo que facilita y hace más rentable la purificación posterior de las proteínas diana.
Experimente las ventajas de Culturefuge: separadores de eyección de sólidos y centrifugadoras de células
- Calidad constante del producto: las condiciones totalmente herméticas eliminan eficazmente la interfaz aire-líquido dentro del recipiente
- Menor riesgo de cizallamiento y contaminación: el exclusivo alimentador de husillo hueco garantiza una separación muy suave de las células
- Los materiales cumplen los requisitos reglamentarios para las industrias biotecnológica y farmacéutica
- Máxima higiene: las boquillas de descarga rocían fuera del recipiente de la centrifugadora y dentro de la campana del bastidor
- Fácil ampliación: el diseño compacto y flexible puede ampliarse con precisión desde la planta piloto hasta la producción
La centrífuga de células Culturefuge de Alfa Laval está disponible como sistema montado sobre patines y esterilizable con vapor. Incluye tuberías para líquidos de servicio y de proceso, junto con un sistema eléctrico integrado que cuenta con un PLC y una unidad neumática. Las opciones de diseño incluyen una superficie de contacto con el producto con un valor Ra <0,5 µm.
Innovative technology behind Culturefuge cell centrifuge
Hermetic Design™
The Hermetic Design™ is a unique concept that provides various benefits for Culturefuge customers. It enables the gentle acceleration of shear-sensitive biological material through a hollow spindle design, minimizing cell lysis and preventing the release of unwanted intracellular proteins or the destruction of fragile protein precipitates. This enhances yields, improves product quality, and optimizes downstream operations. As a fully hermetic separator, it effectively eliminates any air-liquid interface inside the separator bowl, preventing foaming. With this feature, all internal surfaces remain completely wetted, ensuring efficient CIP. Additionally, flow control devices can be positioned downstream, allowing for the implementation of an obstruction-free, full-bore feed arrangement.
UniDisc™
Alfa Laval UniDisc™ increases disc area for improved separation capacity by up to 30%, while at the same time providing an easy to clean design and never-before-seen degree of hygiene.
Bactofuge®
Bactofuge® enables gentle and speedy processing of high-solids broths, resulting in higher yield, with minimum risk of clogging. In addition to higher separation output, Bactofuge preserves the quality of the solids stream, and allows a flexible and controlled operation. Available in select models of the portfolio.
How the cell culture centrifuge works?
The Culturefuge cell separator provides gentle, fully hermetic treatment of cell cultures and other shear-sensitive biological materials. The biological material enters the cell centrifuge through a hollow spindle feed inlet and gradually accelerates as it moves upward to the disc stack, where separation occurs in the rotating centrifuge bowl. The separated liquid phase exits through the liquid outlet at the top of the bowl, while the collected solids are intermittently discharged from the periphery of the bowl. During normal operation, operating water keeps the sliding bowl bottom closed against the bowl hood. During discharge, the sliding bowl bottom drops for a fraction of a second, allowing the solids to be ejected through the discharge ports.
Scalable cell culture centrifuge
Ideal for cell culture fermentation and continuous cell harvesting under hermetic conditions, the Culturefuge cell centrifuge delivers consistent product quality. In addition, it can be accurately and reliably scaled up from use in pilot plants to full-scale production.
Hygienic design
Culturefuge cell centrifuges are made with high-quality materials that meet strict industry regulations. Thanks to their Hermetic Design, they maintain a fully sealed environment, reducing the risk of contamination and ensuring superior hygiene. They are also designed for easy cleaning and sanitation without the need for complicated disassembly. With Sterilization-in-Place (SIP) and Cleaning-in-Place (CIP) systems, the centrifuge can be cleaned automatically, maintaining a hygienic process. Additionally, flushing nozzles spray water inside and outside key components—like the centrifuge bowl and frame hood—for maximum cleanliness.
Complete separation system
The Alfa Laval Culturefuge cell centrifuge comes as a ready-to-use, skid-mounted system. It includes a disk-stack separator on a sturdy base with a horizontal drive shaft, worm gear, lubricating oil bath, and hollow vertical bowl spindle. The system also features piping for service and process liquids. For easy operation, it comes with a built-in electrical system, including a starter, programmable logic control (PLC), and a pneumatic unit. The separator is designed to meet major pressure vessel standards, including ASME and PED, ensuring safety and compliance with industry regulations.
The Culturefuge range
Culturefuge 100
- For pilot plants and small-scale production
- Capacities from 200 to 2,000 l/hr
Culturefuge 200
- For medium scale production with capacities ranging from 1,000 to 5,000 l/hr
Culturefuge 300
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For medium to large scale production with capacities ranging from 2,000 to 8,000 l/hr
Culturefuge 400
- For large scale production with capacities ranging from 3,000 l/h to 25,000 l/h
Culturefuge 400B
- Bactofuge®: continuous solids removal for high concentrations cultures
- For large scale production with capacity from 3,000 l/h to 25,000 l/h
Upon request, Alfa Laval can provide validation support services and a witnessed Factory Acceptance Test (FAT) at our in-house testing facility.
Services
Amplía el rendimiento de su equipo
Nuestra red de servicio global especial le proporciona soporte con piezas y conocimiento experto allí donde esté y siempre que lo necesite. Al garantizar un tiempo de operación, una disponibilidad y una optimización mayores, le proporcionamos tranquilidad y maximizamos el retorno de la inversión. Podemos colaborar con usted para integrar nuestros servicios en un acuerdo de servicio de Alfa Laval para que sus operaciones sean predecibles y no le causen preocupaciones.
Test, validate, and scale up your next separation solution
Whether upgrading your production line or running a proof of concept, Alfa Laval helps you choose the right separator solution with confidence. Simply get in touch to arrange a consultation and trial at one of our test centres, or to rent a unit for onsite testing.
The Separator Innovator
Would you like to know more about our innovations in separation technology? Click on the link below. Alfa Laval invented the first disc stack separator and has led the way in the development of centrifuge technology for over a century. We are glad to share with you the expertise we have gained along the way. Visit our Separator Innovator knowledge base to learn more about advancements in separation and the steps Alfa Laval is taking to continue revolutionizing the technology.
